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Fig 3. Effect of ethanolic extract of propolis from P. catamarcensis (EEP-Pc) and T. fiebrigi (EEP-Tf) in N2 C. elegans viability after.

ABSTRACT: (A) 24 h, (B) 48 h, (C) reproduction, and (D) locomotion. Values are expressed as mean ± SEM. ***P <0.001, ****P <0.0001 versus Control.

ORGANISM(S): Caenorhabditis elegans

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PROVIDER: S-BSST1920 | biostudies-other |

REPOSITORIES: biostudies-other

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Project description:ObjectivesThis study was conducted to evaluate the effect of ethanolic extract of propolis on antibacterial and microshear bond strength of glass ionomer restorations to dentin.Materials and methodsConventional glass ionomer cement (Equia forte, GC Tokyo, Japan), resin-modified glass ionomer (Fuji II LC, GC Tokyo, Japan) and propolis powder (dried extract from honey bees) materials were used in this study. Both conventional glass ionomer and resin-modified glass ionomer were modified by two different concentrations of ethanolic extract of propolis (10 % and 25 % EEP). For antibacterial test, Streptococcus mutans strain was spread on agar petri dishes using a sterile swab. Discs of both glass ionomer restorative materials (without adding EEP, with 10 % EEP and with 25 % EEP) were fabricated within the agar plates. Antibacterial activity was evaluated by measuring the inhibition zones around each disc. For microshear bond strength test, 60 healthy human permanent molars were prepared by cutting occlusal surface and expose the dentin at the height of contour of all teeth then conditioned using poly acrylic acid conditioner, both glass ionomer restorative materials (without adding EEP, with 10 % EEP and with 25 % EEP) were mixed and applied on conditioned dentin surface by using tygon tube. Microshear bond strength was evaluated by the universal testing machine.ResultsTwo-way ANOVA test revealed that both glass ionomer type and different concentrations of EEP had significant effect on the antibacterial test results and microshear bond strength values (p < 0,05). Glass ionomer restorative material with 25%EEP had the highest antibacterial values whereas glass ionomer restorative material without modifications (control groups) had the lowest values. Resin-modified glass ionomer without any modification (control group) had the highest bond strength while resin-modified glass ionomer with 25%EEP had the lowest bond strength.ConclusionsIncorporation of ethanolic extract of propolis to glass ionomer restorative material increases the antibacterial effects of both conventional GIC and RMGI. Inspite of this advantage, it seems that it has deleterious effect on microshear bond strength to dentin.

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Fig 3. Effect of ethanolic extract of propolis from P. catamarcensis (EEP-Pc) and T. fiebrigi (EEP-Tf) in N2 C. elegans viability after.

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