Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide CRISPR-Cas9 screen identifies host factors required for influenza virus replication

ABSTRACT: We performed a genome-wide CRISPR knockout screen in human A549 cells to identify host genes required for influenza A virus replication. The dataset includes processed sgRNA count tables (.txt).

ORGANISM(S): Homo sapiens (human)

SUBMITTER: Mark Feng

PROVIDER: S-BSST2144 | biostudies-other |

SECONDARY ACCESSION(S): PRJNA1306789

REPOSITORIES: biostudies-other

ACCESS DATA

Json Xml

Similar Datasets

Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication.

Project description: Not available

| S-EPMC5939577 | biostudies-literature

Genome-Wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication

Project description:The emergence of influenza A viruses (IAV) from zoonotic reservoirs poses a great threat to human health. As seasonal vaccines are ineffective against zoonotic strains, and newly transmitted viruses can quickly acquire drug resistance, there remains a need for host- directed therapeutics against IAV. Here, we performed a genome-scale CRISPR/Cas9 knockout screen in human lung epithelial cells with a human isolate of an avian H5N1 strain. Several genes involved in sialic acid biosynthesis and related glycosylation pathways were highly enriched post-H5N1 selection, including SLC35A1, a sialic acid transporter essential for IAV receptor expression and thus viral entry. Importantly, we have identified capicua (CIC) as a negative regulator of cell intrinsic immunity, as loss of CIC resulted in heightened antiviral responses and restricted replication of multiple viruses. Therefore, our study demonstrates that the CRISPR/Cas9 system can be utilized for the discovery of host factors critical for the replication of intracellular pathogens.

2018-02-28 | GSE111166 | GEO

Genome-wide CRISPR screen identifies host dependency factors for influenza A virus infection.

Project description: Not available

| S-EPMC6952391 | biostudies-literature

Genome-Wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication

Project description:Genome-Wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication

| PRJNA436088 | ENA

Genome-Wide CRISPR Screen Identifies RACK1 as a Critical Host Factor for Flavivirus Replication.

Project description: Not available

| S-EPMC8610583 | biostudies-literature

Human genome-wide RNAi screen reveals host factors required for enterovirus 71 replication.

Project description: Not available

| S-EPMC5071646 | biostudies-literature

Genome-scale CRISPR screen identifies TMEM41B as a multi-function host factor required for coronavirus replication.

Project description: Not available

| S-EPMC8675922 | biostudies-literature

Genome-wide CRISPR screen Identifies Host Dependency Factors for Influenza A Virus Infection

Project description:In this study, we performed a genome-wide CRISPR screen in A549 cells to identify host genes that are required forInfluenza A virus (IAV) infection.Amongst the identified genes, WDR7, CCDC115, TMEM199 and CMTR1 conferred strong protection against IAV infection when they were CRISPR-deleted without impacting host cell fitness. To investigate the roles of these genes on the transciptomic states of the cell, we performed bulk RNA-sequencing of WDR7, CCDC115, TMEM199 and CMTR1 polyclonal knockout cells. We found that cells lacking WDR7, CCDC115 or TMEM199 up-regulates expression of lysosomal genes due to increased nuclear translocation of Transcription factor EB (TFEB), which hampers IAV entry. In addition,we report that CRISPR deletion of CMTR1 results in increased expression of Interferon-stimulated genes (ISGs) that also plays a role inblocking IAV infection.

2019-11-30 | GSE141171 | GEO

A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection.

Project description: Not available

| S-EPMC8893346 | biostudies-literature

A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors.

Project description: Not available

| S-EPMC5511375 | biostudies-literature