Have the cake and eat it: Optimizing nondestructive DNA metabarcoding of macroinvertebrate samples for freshwater biomonitoring

ABSTRACT: DNA metabarcoding can contribute to improving cost?effectiveness and accuracy of biological assessments of aquatic ecosystems, but significant optimization and standardization efforts are still required to mainstream its application into biomonitoring programmes. In assessments based on freshwater macroinvertebrates, a key challenge is that DNA is often extracted from cleaned, sorted and homogenized bulk samples, which is time?consuming and may be incompatible with sample preservation requirements of regulatory agencies. Here, we optimize and evaluate metabarcoding procedures based on DNA recovered from 96% ethanol used to preserve field samples and thus including potential PCR inhibitors and nontarget organisms. We sampled macroinvertebrates at five sites and subsampled the preservative e

ORGANISM(S): benthic macroinvertebrates

SUBMITTER: Filipa Matos Silva Martins 

PROVIDER: S-BSST241 | biostudies-other |

SECONDARY ACCESSION(S): https://www.ebi.ac.uk/ena/data/view/PRJEB31133

REPOSITORIES: biostudies-other