Cisplatin and the curcuminoid, EF24, modulate distinct apoptosis pathways in ovarian cancer, auditory and renal cell lines

ABSTRACT: Abstract Purpose To determine whether the curcuminoid (3E,5E)-3,5-bis[(2-fluorophenyl) methylene]-4-piperidinone (EF24) increases the anti-cancer effects of cisplatin against the human ovarian cancer cell line, A2780, and the cisplatin resistant human ovarian cancer line, A2780cis, and to determine whether EF24 prevents cisplatin-mediated side effects in the mouse auditory hybridoma cell line, HEI-OC1, and the human kidney cell line, HEK-293T. Methods The effect of cisplatin and EF24 on cellular viability was measured using the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Reactive oxygen species (ROS) production was measured using flow cytometry. The expression and activity of apoptosis signal transduction proteins (apoptosis inducing factor

SUBMITTER: Michael E. Smith 

PROVIDER: S-BSST311 | biostudies-other |

REPOSITORIES: biostudies-other