Project description:The whole-genome oligonucleotide microarray analysis of NS398-treated HT29 colon adenocarcinoma cells samples can give an insight into global molecular background of selective COX2 inhibitor administration in order to find other target molecules and pathways influenced by NS398 selective COX2 inhibitor treatment in the epithelial cells.
Project description:CCDC12 is a newly discovered potential oncogene by our team, and it is proposed to observe the molecules that have interaction with CCDC12 by the technology of proteomic sequencing to map the interaction network of CCDC12. The refined molecular interaction network will help to better observe the role of CCDC12 in cancer for targeted modulation. In this study, we used 4plex iTRAQ technology to sequence proteins from colon cancer cells overexpressing CCDC12, and finally found the differential proteins.
Project description:The whole-genome oligonucleotide microarray analysis of NS398-treated HT29 colon adenocarcinoma cells samples can give an insight into global molecular background of selective COX2 inhibitor administration in order to find other target molecules and pathways influenced by NS398 selective COX2 inhibitor treatment in the epithelial cells. Total RNA was extracted from NS398-treated and control HT29 colon adenocarcinoma cells and hybridized on Affymetrix HGU133 Plus 2.0 microarrays