Transcription profiling of human breast cancer cell lines (MCF-7 and MDA-MB-231)

ABSTRACT: We used microarrays to detail the global programme of gene expression for MCF-7 and MDA-MB-231 and revealed the correlation between the methylation state of various genomic components and gene expression level. The expression analyses of the two breast cancer cell lines are a part of the whole study. The summary of our study is as follows: We establish a technique, called modified methylation-specific digital karyotyping (MMSDK) based on methylation-specific digital karyotyping (MSDK) with a novel sequencing approach. Briefly, after a tandem digestion of genomic DNA with a methylation-sensitive mapping enzyme and a fragmenting enzyme, short sequence tags are obtained. These tags are amplified, followed by direct, massively parallel sequencing (Solexa 1G Genome Analyzer). This method allows

ORGANISM(S): Homo sapiens

SUBMITTER: Li Jian 

PROVIDER: S-ECPF-GEOD-12199 | biostudies-other |

REPOSITORIES: biostudies-other