Transcription profiling of human uterine leiomyomata - identifying the molecular signature of the interstitial del(7q) subgroup using a paired analysis

ABSTRACT: Uterine leiomyomata (UL), the most common neoplasm in reproductive-age women, have recurrent cytogenetic abnormalities including del(7)(q22q32). To develop a molecular signature, matched del(7q) and non-del(7q) tumors identified by FISH or karyotyping from 11 women were profiled with expression arrays. Our analysis using paired t-tests demonstrates this matched design is critical to eliminate confounding effects of genotype and environment that underlie patient variation. A gene list ordered by genome-wide significance showed enrichment for the 7q22 target region. Modification of the gene list by weighting each sample for percent of del(7q) cells to account for the mosaic nature of these tumors further enhanced the frequency of 7q22 genes. Pathway analysis revealed two of the 19 signi

ORGANISM(S): Homo sapiens

SUBMITTER: Hodge JC 

PROVIDER: S-ECPF-GEOD-12814 | biostudies-other | 2009 Oct

REPOSITORIES: biostudies-other