Tamoxifen-resistant human breast cancer cell lines

ABSTRACT: A functional genetic screen to identify genes causing tamoxifen resistance in an estrogen-dependent human breast cancer cell model was performed. By insertion of defective retrovirus into the genome, individual genetic changes were introduced at random in ZR-75-1 cells. Subsequently, infected cells were selected for their ability to proliferate while being exposed to tamoxifen, and from these cultures stable cell lines were established. The retrovirus insertion sites were mapped enabling the identification of several candidate breast cancer anti-estrogen resistance (BCAR) genes. By cDNA transfection of estrogen-dependent cells resulting in their transformation into a tamoxifen-resistant phenotype, proof was provided for the causative role of BCAR genes.A functional genetic screen to identi

ORGANISM(S): Homo sapiens

SUBMITTER: van Agthoven T 

PROVIDER: S-ECPF-GEOD-14513 | biostudies-other | 2009 Dec

REPOSITORIES: biostudies-other