Electric pulses do not change expression profile of genes involved in development of cancer in malignant melanoma cells

ABSTRACT: Electroporation is a versatile method for in vitro or in vivo delivery of different molecules into cells. However, no study so far has analysed the effects of electric pulses used in electrochemotherapy (ECT pulses) or electric pulses used in electrogene therapy (EGT pulses) on malignant cells. We studied the effect of ECT and EGT pulses in human malignant melanoma cells in vitro in order to understand and predict possible effect of electric pulses on gene expression and their possible effect on cell behaviour. We used microarrays with 2698 different oligonucleotides to obtain expression profile of genes involved in apoptosis and development of cancer in a malignant melanoma cell line (SK-MEL28) exposed to ECT pulses and EGT pulses. Cells exposed to ECT pulses showed 68.8% average survival

ORGANISM(S): Homo sapiens

SUBMITTER: Mlakar V 

PROVIDER: S-ECPF-GEOD-15420 | biostudies-other | 2009

REPOSITORIES: biostudies-other