Transcription by array of human buccal mucosa from smokers and non-smokers

ABSTRACT: A tissue like buccal mucosa (from cheek swabs) would be an ideal sample material for rapid, easy collection for testing of biomarkers as an alternative to blood. A limited number of studies, primarily in the smoker/oral cancer literature, address this tissue's efficacy for quantitative PCR or microarray gene expression analysis. In this study both qPCR and microarray analyses were used to evaluate gene expression in buccal cells. An initial study comparing blood and buccal cells from the same individuals looked at relative amounts of four genes. The RNA isolated from buccal cells was degraded but was of sufficient quality to be used with RT-qPCR to detect expression of specific genes. Second, buccal cell RNA was used for microarray-based differential gene expression studies by comparin

ORGANISM(S): Homo sapiens

SUBMITTER: Kupfer DM 

PROVIDER: S-ECPF-GEOD-16149 | biostudies-other | 2010

REPOSITORIES: biostudies-other