Browse
Submit Data
Databases
API
Help

Dataset Information

7 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

NP-18 vs. NP-18AR transcriptional profile upon treatment with TK/GCV

ABSTRACT: Pancreatic cancer-derived cells NP-18 underwent four rounds of treatment with increasing doses of an adenoviral vector encoding TK enzyme and GCV. Surviving cells were termed NP-18AR and displayed decreased sensitivity to treatment. This experiment analyses the transcriptomic effect of TK/GCV treatment on NP-18AR as compared to that of NP-18 cells. Two-condition experiment, where response to treatment in naïve cells (NP-18) was compared to response to treatment in previously treated resistant cells (NP-18AR). Biological replicates: samples from 3 independently generated and independently treated NP-18AR lines (NP-18AR1, NP-18AR2 and NP-18AR3) were hibridized with 3 independently treated samples of NP-18 cells. A total of 9 hybridizations were performed, 2 for NP-18AR1 (including 1 dye-swap), 4 for NP-18AR2 (including 2 dye swaps) and 3 for NP-18AR3 (including 1 dye-swap).

ORGANISM(S): Homo sapiens

SUBMITTER: Abate-Daga D

PROVIDER: S-ECPF-GEOD-17137 | biostudies-other | 2010 Oct

REPOSITORIES: biostudies-other

ACCESS DATA

Json Xml

Similar Datasets

NP-18 vs. NP-18AR transcriptional profile upon treatment with TK/GCV

2010-07-16 | GSE17137 | GEO

NP-18 vs. NP-18AR transcriptional profile upon treatment with TK/GCV

Project description:Pancreatic cancer-derived cells NP-18 underwent four rounds of treatment with increasing doses of an adenoviral vector encoding TK enzyme and GCV. Surviving cells were termed NP-18AR and displayed decreased sensitivity to treatment. This experiment analyses the transcriptomic effect of TK/GCV treatment on NP-18AR as compared to that of NP-18 cells. Two-condition experiment, where response to treatment in naïve cells (NP-18) was compared to response to treatment in previously treated resistant cells (NP-18AR). Biological replicates: samples from 3 independently generated and independently treated NP-18AR lines (NP-18AR1, NP-18AR2 and NP-18AR3) were hibridized with 3 independently treated samples of NP-18 cells. A total of 9 hybridizations were performed, 2 for NP-18AR1 (including 1 dye-swap), 4 for NP-18AR2 (including 2 dye swaps) and 3 for NP-18AR3 (including 1 dye-swap).

2010-07-16 | E-GEOD-17137 | biostudies-arrayexpress

Robust learning for optimal treatment decision with NP-dimensionality.

Project description: Not available

| S-EPMC5544015 | biostudies-literature

Metabolomics analysis of liver reveals profile disruption in bovines upon steroid treatment

Project description:INTRODUCTION: The surveillance of illegal anabolic practices in bovine meat production is necessary to guarantee consumers’ health. Screening strategies based on the recognition of indirect biological effects are considered by the community as promising tools to overcome some limitations of classical analytical methods and might therefore concur to ensure safer food for the consumer.OBJECTIVES: The present work aims at characterizing the metabolic profile induced in liver by administration of anabolic steroids, and at identifying potential disturbances in the hepatic metabolism.METHODS: A total of 32 liver samples, 16 from untreated bulls and 16 from bulls treated with an ear implant (Revalor-XS®) containing trenbolone acetate (200 mg) and ß-estradiol (40 mg), were analyzed following a LC–MS-based metabolomic analysis combining RP and HILIC chromatographic separations. Different multivariate statistical tools were applied to the datasets to select common metabolites that may be considered as potential markers based on their significant changes in concentrations after administration of sexual steroids.RESULTS:Eight candidate markers were identified. Moreover, a subset of four markers was also validated by a different laboratory that performed the same analysis using an independent instrumental and elaboration platform, confirming the robustness of the results achieved.CONCLUSION: This study was performed mimicking experimental conditions that may be used during a potential misuse practice. It is promising in the objective of setting up an analytical strategy to highlight sexual steroids abuse in livestock animals.

2018-05-08 | MTBLS436 | MetaboLights

T1E-18 vs T1E-18 Indole

Project description:Transcriptional profile of Pseudomonas putida DOT-T1E-18 strain grown in the presence of indole (300 uM) compared with the same cells grown in absence of indole.

2013-03-15 | E-MEXP-3822 | biostudies-arrayexpress

T1E-18 vs T1E-18 Ampicillin

Project description:Transcriptional profile of Pseudomonas putida DOT-T1E-18 grown in the presence of ampicillin (100 ug/ml) compared with the same cells grown in absence of ampicillin.

2013-03-15 | E-MEXP-3824 | biostudies-arrayexpress

Two experts and a newbie: [18F]PARPi vs [18F]FTT vs [18F]FPyPARP-a comparison of PARP imaging agents.

Project description: Not available

| S-EPMC8803746 | biostudies-literature

Methylome analysis of 18 wild-type and mutant mouse ES, NP and MEF cells

Project description:The analysis of the 18 methylomes comprise 3 biological replicates of TDG+/- ES cells, 3 biological replicates of TDG-/- ES cells, 3 biological replicates of TDG+/- NP cells, 3 biological replicates of TDG-/- NP cells, 3 biological replicates of TDG+/+ MEF cells and 3 biological replicates of TDG-/- MEF cells.

2011-02-24 | GSE27468 | GEO

Expression profile of 22Rv1 cells upon DHT treatment

Project description:To determine genes regulated by Androgen Receptor in response to DHT treatment in 22Rv1, we cultured 22Rv1 cells in full serum containing media and then added DHT for 24 hrs. RNA was harvested after 24 hrs of treatment and gene expression profiling was performed by RNA-Seq

2017-11-01 | GSE86503 | GEO

bre1 vs WT transcriptional profile

Project description:Relative mRNA abundance of all S. cerevisiae genes was measured in bre1 compared to wild-type.

2009-11-19 | GSE13701 | GEO