HuR differentially regulates unique subsets of mRNAs in estrogen receptor negative and positive breast cancer

ABSTRACT: Analysis of RNA immunoprecipitation of HuR, a RNA binding protein (RBP), in breast cancer cell lines. This approach, utilizing RNA immunoprecipitation hybridized to microarray (RIP-Chip), provides global identification of putative endogenous mRNA targets of different RBPs. HuR is an RBP that binds to the AU-rich (ARE) regions of labile mRNAs, such as proto-oncogenes, facilitating their translation into protein. HuR has been shown to play a role in cancer progression and elevated levels of cytoplasmic HuR directly correlate with increased invasiveness and poor prognosis for many cancers, including those of the breast. We used HuR RIP-Chip as a comprehensive and systematic method to survey breast cancer target genes in both MCF-7 (estrogen receptor positive, ER+) and MDA-MB-231 (estrogen r

ORGANISM(S): Homo sapiens

SUBMITTER: Calaluce R 

PROVIDER: S-ECPF-GEOD-17820 | biostudies-other | 2010

REPOSITORIES: biostudies-other