Development of a novel splice array platform and its application in the identification of alternative splice variants

ABSTRACT: Changes in alternative splicing are associated with several pathological conditions, including cancer. Microarrays strategies, which allow for the characterization of thousands of alternative splice forms in a single test, can be applied to identify differential alternative splicing events. In this study, a novel splice array platform was developed, including the design of a high-density oligonucleotide array, a labeling procedure, and an algorithm to identify splice events. The array consists of exon probes and thermodynamically balanced junction probes. Suboptimal probes are tagged and considered in the final analysis. An unbiased labeling protocol was developed using random primers. The algorithm used to distinguish changes in expression from changes in splicing was calibrated using int

ORGANISM(S): Homo sapiens

SUBMITTER: Blanco David 

PROVIDER: S-ECPF-GEOD-18346 | biostudies-other |

REPOSITORIES: biostudies-other