Pancreatic cancercirculating tumor cells express a cell motility gene signature that predicts survival after surgery

ABSTRACT: Most cancer deaths are caused by metastases, which are the end-results of circulating tumor cells (CTC) that detach from the cancer primary and succeed to survive in distant organs. The aim of the present study was to develop a gene signature of CTC and to assess its prognostic relevance after surgery for pancreatic ductaladenocarcinoma (PDAC). A negative depletion fluorescence activated cell sorting (FACS) procedure was developed and validated with spiking experiments using cancer cell lines in whole human blood samples. This FACS-based method was used to enrich for CTC from the blood of 10 patients who underwent surgery for PDAC. Total RNA was isolated from 4 subgroup samples, i.e. CTC, haematological cells (G), original tumor (T), and non-tumoral pancreatic control tissue (P). After RNA

ORGANISM(S): Homo sapiens

SUBMITTER: Sergeant G 

PROVIDER: S-ECPF-GEOD-18670 | biostudies-other | 2012

REPOSITORIES: biostudies-other