DUSP1/MKP1 promotes angiogenesis, invasion and metastasis in non-small cell lung cancer

ABSTRACT: DUSP1 is involved in different cellular pathways including cancer cell proliferation, angiogenesis, invasion and resistance to chemotherapy. To understand more about the cellular responses regulated by DUSP1 in NSCLC cells, we interfered DUSP1 expression in the NSCLC cell line H460 and studied the changes in gene expression differentially regulated by this phosphatase. Keywords: Expression profiling by array in cells with genetic modification The human non small lung cancer cell line H460 used in this study was from ATCC (American Type Culture Collection). The cell line was maintained in RPMI (Gibco, Invitrogen) supplemented with 10% bovine serum and transfected by Lipofectamine Plus Reagent from Life Technologies as directed by the manufacturer. The DUSP1 pSuperRetro-derived vectors were

ORGANISM(S): Homo sapiens

SUBMITTER: Moncho-Amor V 

PROVIDER: S-ECPF-GEOD-19592 | biostudies-other | 2011 Feb

REPOSITORIES: biostudies-other