Expression profiling of BRAFV600E melanoma cell lines upon RAF inhibition

ABSTRACT: Microarray expression analysis to identify global changes in transcription in response to RAF inhibition. Genes under RAF control were identified in a panel of BRAFV600E tumor cells, following the short-term inhibition of RAF using a pan-RAF kinase inhibitor, PLX4032 (Plexxikon). For comparison with changes in gene expression in response to MEK inhibition using PD0325901 (Pfizer), the following array data was referenced: (http://www.ncbi.nlm.nih.gov/geo/ (accession no. GSE10086)). Cell lines growing in culture (n=5) were treated with the RAF inhibitor PLX4032 (250nM or 1000nM) or vehicle alone (0.1% DMSO) as control, for eight hours.

ORGANISM(S): Homo sapiens

SUBMITTER: Joseph EW 

PROVIDER: S-ECPF-GEOD-20051 | biostudies-other | 2010 Aug

REPOSITORIES: biostudies-other