Estrogen-mediated Epigenetic Repression of Large Chromosomal Regions through DNA Looping

ABSTRACT: The current concept of epigenetic repression is based on one repressor unit corresponding to one silent gene. This notion, however, cannot adequately explain concurrent silencing of multiple loci observed in large chromosome regions. The long-range epigenetic silencing (LRES) can be a frequent occurrence throughout the human genome. To comprehensively characterize the influence of estrogen signaling on LRES, we analyzed transcriptome, methylome, and estrogen receptor alpha (ESR1)-binding datasets from normal breast epithelia and breast cancer cells. This ?omics? approach uncovered 11 large repressive zones (range: 0.35~5.98 megabases), including a 14-gene cluster located on 16p11.2. In normal cells, estrogen signaling induced transient formation of multiple DNA loops in the 16p11.2 region

ORGANISM(S): Homo sapiens

SUBMITTER: Hsu PY 

PROVIDER: S-ECPF-GEOD-21068 | biostudies-other | 2010 Jun

REPOSITORIES: biostudies-other