Transcriptional and post-transcriptional regulation of VEGF by the unfolded protein response

ABSTRACT: Inadequate extracellular conditions can adversely affect the environment of the ER and impinge on the maturation of nascent proteins. The resultant accumulation of unfolded proteins activates a signal transduction pathway, known as the unfolded protein response, which serves primarily to protect the cell during stress and helps restore homeostasis to the ER. Microarray analysis of the unfolded protein response in a human medulloblastoma cell line treated with thapsigargin revealed that, in addition to known targets, a large number of proangiogenic factors were up-regulated. Real-time PCR analyses confirmed that four of these factors, VEGF, FGF2, angiogenin and IL-8, were transcriptionally up-regulated in multiple cell lines by various ER stress inducers. Our studies on VEGF regulation r

ORGANISM(S): Homo sapiens

SUBMITTER: Pereira ER 

PROVIDER: S-ECPF-GEOD-21979 | biostudies-other | 2010

REPOSITORIES: biostudies-other