Luminal-like and basal-like in vivo breast cancer xenograft models

ABSTRACT: The number of relevant and well-characterized cell lines and xenograft models for studying human breast cancer are few, and may represent a limitation for this field of research. With the aim of developing new breast cancer model systems for in vivo studies of hormone dependent and independent tumor growth, progression and invasion, and for in vivo experimental therapy studies, we collected primary mammary tumor specimens from patients, and implanted them in immunodeficient mice. Primary tumor tissue from 29 patients with breast cancer was implanted subcutaneously with matrigel in SCID mice, in the presence of continuous release of estradiol. The tumors were transferred into new animals when reaching a diameter of 15mm and engrafted tumors were harvested for morphological and molecular cha

ORGANISM(S): Homo sapiens

SUBMITTER: Bergamaschi A 

PROVIDER: S-ECPF-GEOD-23041 | biostudies-other | 2009 Dec

REPOSITORIES: biostudies-other