ZNF703 overexpression and/or knockdown in the breast cancer cell line MCF-7

ABSTRACT: The telomeric amplicon at 8p12 is common in ER+ breast cancers. Array-CGH and expression analyses of 1172 tumors revealed ZNF703/Zeppo1 was the single gene within the minimal amplicon and was amplified predominantly in the Luminal B subtype. Amplification was shown to correlate with increased gene and protein expression and was associated with a distinct expression signature and poor outcome. In the luminal MCF-7 cell line manipulation of ZNF703 expression altered transcription of genes also present within the primary tumor signature, including TGFBR2 (whose promoter was bound by ZNF703). Overexpression of ZNF703 rendered MCF-7 cells insensitive to TGFβ-induced suppression of mammosphere formation. Forced overexpression of ZNF703 in normal human breast epithelial cells enhanced the frequen

ORGANISM(S): Homo sapiens

SUBMITTER: Holland DG 

PROVIDER: S-ECPF-GEOD-23673 | biostudies-other | 2011 Mar

REPOSITORIES: biostudies-other