Cell-specific and spatio-temporal controls of the estrogen-responsive trefoil factor (TFF) locus activity.II

ABSTRACT: Cell-specific transcriptional regulations exerted by the estrogen (E2) receptor alpha (ER) heavily rely upon timely and spatially coordinated processes. We engaged a comparative analysis of such dynamic molecular events at the TFF locus harbouring a cluster of genes co-regulated by E2, in two distinct breast cancer cell lines. Using a combination of methods, we show that the recruitment of ER on cell-specific sites triggers dynamic local modifications of chromatin, which are coordinated in time all along the locus. DNA-FISH experiments further demonstrate that these changes are associated with an E2-dependent reduction in plasticity of this genomic region and are dependent upon cohesin. Importantly, 3C/4C experiments and the use of triplex forming oligonucleotides (TFOs) allowed us to prec

ORGANISM(S): Homo sapiens

SUBMITTER: Quintin J 

PROVIDER: S-ECPF-GEOD-23850 | biostudies-other | 2014 Jul

REPOSITORIES: biostudies-other