MKK2 is sufficient but not necessary for proliferation and anchorage-independent growth of SK-MEL-28 cells

ABSTRACT: Though mitogen activated protein kinase kinases (MKK or MEK) 1 and 2 are widely assumed to be functionally redundant some reports indicate they possess distinct biologic activities. To test the hypothesis that MEK1 and MEK2 signaling pathways are interchangeable we used two complementary approaches to determine the necessity and sufficiency of individual MEK1 and MEK2 signaling pathways for human melanoma SK-MEL-28 cell proliferation. To test the necessity we targeted MEK1 and/or MEK2 using specific siRNAs. An effect on proliferation was observed only when both MEK1 and MEK2 were knocked down indicating that neither of the individual MEK isoforms is necessary for SK-MEL-28 cell proliferation. To test the sufficiency we inhibited multiple MEK and MKK signaling pathways in SK-MEL-28 cell

ORGANISM(S): Homo sapiens

SUBMITTER: Lee CS 

PROVIDER: S-ECPF-GEOD-23930 | biostudies-other | 2011

REPOSITORIES: biostudies-other