Expression data from tumor and normal epithelium derived from BRCA1-mutation carriers

ABSTRACT: Microarrays were used to determine relative global gene expression changes in WT and BRCA1-mutation carrier breast epithelium as well as tumors created from WT and BRCA1-mutation carrier breast epithelial cells. Total RNA was isolated from freshly dissociated mammary epithelilal cells obtained from disease-free prophylactic masectomy tissues of 4 different BRCA1-mutation carriers or 4 different reduction mammoplasty tissues from non-mutation carriers. Total RNA was also isolated from fresh tumor tissues derived from in vivo transformed human mammary epithelial cells created from cells obtained from WT or BRCA1-muation carrires. Dissociated mammary epithelial cells were transduced with lentiviruses encoding mutant p53, mutant ras, mutant PI3K and cyclin D1 and injected into in humanized gl

ORGANISM(S): Homo sapiens

SUBMITTER: Proia TA 

PROVIDER: S-ECPF-GEOD-25835 | biostudies-other | 2011 Feb

REPOSITORIES: biostudies-other