Activator protein-2? is a critical determinant of estrogen receptor interactome formation and gene transcription in breast cancer [expression]

ABSTRACT: Estrogen receptor α (ERα) is key player in the progression of breast cancer. ERα binds to DNA and mediates long-range chromatin interactions throughout the genome, but the underlying mechanism in this process is unclear. Here, we show that AP-2 motifs are highly enriched in the ERα binding sites (ERBS) identified from the recent ChIA-PET of ERα. More importantly, we demonstrate that AP-2γ (also known as TFAP2C), a member of the AP-2 family which has been implicated in breast cancer oncogenesis, is recruited to chromatin in a ligand-independent manner and co-localized with ERα binding events. Furthermore, pertubation of AP-2γ expression disrupts ERα DNA binding, long-range chromatin interactions, and gene transcription. Using ChIP-seq, we show that AP-2γ and ERα binding occurs in close prox

ORGANISM(S): Homo sapiens

SUBMITTER: Tan SK 

PROVIDER: S-ECPF-GEOD-26740 | biostudies-other | 2011 Jul

REPOSITORIES: biostudies-other