Differential expression of microRNAs between oral squamous cell carcinoma and healthy control tongues

ABSTRACT: In order to identify aberrantly expressed microRNAs in oral squamous cell carcinomas (OSCCs), we have employed microRNA microarray profile using healthy tongue samples as control. All seventeen human OSCCs and three normal tongue tissues were collected from the Tissue Bank at the Moffitt Cancer Center and approved by the University of Florida institutional review board. Majority (15 out of 17) of the OSCCs were derived from tongue cancers. The tumor samples contained greater than 80% of cancerous cells, confirmed by microscopic examination by a head and neck pathologist. The normal tongues were taken from a non-cancerous region. Tissues were snap-frozen and stored at -80°C until further use. Total RNAs were isolated from human tissues using mirVana miRNA Isolation kit (Ambion/Applied Biosy

ORGANISM(S): Homo sapiens

SUBMITTER: Jung HM 

PROVIDER: S-ECPF-GEOD-28100 | biostudies-other | 2012 Aug

REPOSITORIES: biostudies-other