Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells

ABSTRACT: HOX genes encode a family of homeodomain-containing transcription factors involved in the determination of cell fate and identity during embryonic development. They also behave as oncogenes in some malignancies. In this study, we found high expression of the HOXD9 gene transcript in glioma cell lines and human glioma tissues by quantitative real-time PCR. Using immunocytochemistry, we observed HOXD9 protein expression in human brain tumor tissues, including astrocytomas and glioblastomas. To investigate the role of HOXD9 in gliomas, we silenced its expression in the glioma cell line U87 using HOXD9-specific siRNA, and observed decreased cell proliferation, cell cycle arrest, and induction of apoptosis. It was suggested that HOXD9 contributes to both cell proliferation and/or cell survival.

ORGANISM(S): Homo sapiens

SUBMITTER: Tabuse M 

PROVIDER: S-ECPF-GEOD-28618 | biostudies-other | 2011

REPOSITORIES: biostudies-other