Normal human osteoblasts and MDA-MB-231 mono and coculture

ABSTRACT: As a model for investigating changes in gene expression in response to epithelial-osteoblast interactions in bone metastases of breast carcinomas, cells that represented malignant epithelial cell compartments (MDA-MB-231) and cells that represented skeletal compartments (Normal Human (NH) Osteoblasts) were examined in an in vitro mixed co-culture setting. These two types of cells were co-cultivated for 48 h in a low-serum medium [0.2% fetal bovine serum (FBS)] to allow reciprocal signal exchange with minimal background from undefined molecular signals that are inherent in fetal bovine serum. We examined the effects of co-cultivation on each cell pairing in two independent biological replicates. The gene expression profiles of the co-cultures were compared to the expression profiles of the

ORGANISM(S): Homo sapiens

SUBMITTER: Rajski M 

PROVIDER: S-ECPF-GEOD-29033 | biostudies-other | 2012

REPOSITORIES: biostudies-other