Transcription profiling of human B-cell lymphoma cell lines treated with motexafin gadolinium and zinc reveals induced oxidative stress responses and apoptosis

ABSTRACT: "There is an emerging appreciation of the importance of zinc in regulating cancer cell growth and proliferation. Recently, we showed that the anticancer agent motexafin gadolinium (MGd) disrupted zinc metabolism in A549 lung cancer cells, leading, in the presence of exogenous zinc, to cell death. Here, we report the effect of MGd and exogenous zinc on intracellular levels of free zinc, oxidative stress, proliferation, and cell death in exponential phase human B-cell lymphoma and other hematologic cell lines. We find that increased levels of oxidative stress and intracellular free zinc precede and correlate with cell cycle arrest and apoptosis. To better understand the molecular basis of these cellular responses, gene expression profiling analyses were conducted on Ramos cell cultures treat

ORGANISM(S): Homo sapiens

SUBMITTER: Lecane PS 

PROVIDER: S-ECPF-GEOD-2964 | biostudies-other | 2005 Dec

REPOSITORIES: biostudies-other