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Cancer-related epigenome changes associated with reprogramming to induced pluripotent stem cells

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE29870: Cancer-related epigenome changes associated with reprogramming to induced pluripotent stem cells (expression data) GSE29872: Cancer-related epigenome changes associated with reprogramming to induced pluripotent stem cells (methylation data) Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: Ohm JE

PROVIDER: S-ECPF-GEOD-29873 | biostudies-other | 2010 Oct

REPOSITORIES: biostudies-other

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Project description: Not available

| S-EPMC2980296 | biostudies-literature

Project description:This SuperSeries is composed of the following subset Series: GSE29870: Cancer-related epigenome changes associated with reprogramming to induced pluripotent stem cells (expression data) GSE29872: Cancer-related epigenome changes associated with reprogramming to induced pluripotent stem cells (methylation data) Refer to individual Series

2011-06-16 | E-GEOD-29873 | biostudies-arrayexpress

Project description:This SuperSeries is composed of the SubSeries listed below.

2011-06-16 | GSE29873 | GEO

Project description:The ability to induce pluripotent stem cells from committed, somatic human cells provides tremendous potential for regenerative medicine. However, there is a defined neoplastic potential inherent to such reprogramming that must be understood and may provide a model for understanding key events in tumorigenesis. Using genome-wide assays, we identify cancer-related epigenetic abnormalities that arise early during reprogramming and persist in induced pluripotent stem cell (iPS) clones. These include hundreds of abnormal gene silencing events, patterns of aberrant responses to epigenetic-modifying drugs resembling those for cancer cells, and presence in iPS and partially reprogrammed cells of cancer-specific gene promoter DNA methylation alterations. Our findings suggest that by studying the process of induced reprogramming, we may gain significant insight into the origins of epigenetic gene silencing associated with human tumorigenesis, and add to means of assessing iPS for safety. Direct expression comparison of iPS lines, cultured stem cell lines and normal differentiated cells. Re-expression experiments with 5-aza-2′-deoxycytidine (AZA) and trichostatin A (TSA) to identify hypermethylated genes.

2011-06-16 | E-GEOD-29870 | biostudies-arrayexpress

Project description:he ability to induce pluripotent stem cells from committed, somatic human cells provides tremendous potential for regenerative medicine. However, there is a defined neoplastic potential inherent to such reprogramming that must be understood and may provide a model for understanding key events in tumorigenesis. Using genome-wide assays, we identify cancer-related epigenetic abnormalities that arise early during reprogramming and persist in induced pluripotent stem cell (iPS) clones. These include hundreds of abnormal gene silencing events, patterns of aberrant responses to epigenetic-modifying drugs resembling those for cancer cells, and presence in iPS and partially reprogrammed cells of cancer-specific gene promoter DNA methylation alterations. Our findings suggest that by studying the process of induced reprogramming, we may gain significant insight into the origins of epigenetic gene silencing associated with human tumorigenesis, and add to means of assessing iPS for safety. Methylation was analyzed using Illumina's 27k Infinium platform for direct detection of methylation after bisulfite conversion. The overall methylation status was determined for several iPS lines and the pool cells from which they are derived. These methylation levels can be compared directly to those of cultured stem cells, differentiated cells and cancer cell lines.

2011-06-16 | E-GEOD-29872 | biostudies-arrayexpress

2011-06-16 | GSE29870 | GEO

2011-06-16 | GSE29872 | GEO

Reprogramming of Cancer Cells into Induced Pluripotent Stem Cells Questioned.

Project description: Not available

| S-EPMC6881048 | biostudies-literature

Project description:To identify hypermethylated genes in induced pluripotent stem cell lines, human embryonic stem cell lines, and non-nucleofected CD34+ CB cells, indicated cell lines were treated with 5-aza-2′-deoxycytidine (DAC) or trichostatin A (TSA), and drug-induced changes in gene expression vs control treatment (PBS) were identified using Agilent two-color arrays, using the experimental methods of Ohm et al. (Cancer Res 70:7662-7673 2010).

2017-12-31 | GSE44941 | GEO

Reprogramming of pancreatic beta cells into induced pluripotent stem cells.

Project description: Not available

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