Investigate the anti-tumor mechanisms of But4ManNAc and butyrate in two breast cancer cell lines

ABSTRACT: But4ManNAc - an analog of ManNAc, the committed precursor of sialic acid - decreases metastatic potential, inhibits growth, and triggers apoptosis in many cancer cells. The anti-tumor effects of But4ManNAc may result from a synergistic interaction between butyrate-induced changes in gene expression and ManNAc-induced altered sialylation. This experiment provides insight into synergistic interactions by identifying changes in gene expression specific to But4ManNAc and not shared with butyrate. Specifically, microarrays will compare gene expression of metastatic breast cancer cells (MDA-MB-231) treated with But4ManNAc to that of cells treated with sodium butyrate at concentrations which inhibit invasiveness but do not trigger apoptosis. Differences in gene expression demonstrate a synergi

ORGANISM(S): Homo sapiens

SUBMITTER: Head Steven 

PROVIDER: S-ECPF-GEOD-29922 | biostudies-other |

REPOSITORIES: biostudies-other