MiR-34a targets PDGFRA in proneural malignant glioma
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ABSTRACT: To identify potential targets of miR-34a, we performed transcriptional profiling on proneural TS543 GBM cells, focusing on mRNAs whose levels decreased in response to miR-34a transfection as compared to control oligonucleotide. Proneural TS543 GBM cells were transfected with 100 nM hsa-miR-34a or control oligonucleotide using Hiperfect transfection reagent (Qiagen). After 3 days, RNA was isolated and expression analyses were performed using Illumina HT-12 bead array. The microarray dataset was normalized using a variance stable normalization (VSN) procedure in the ‘lumi’ package from the Bioconductor framework.
ORGANISM(S): Homo sapiens
SUBMITTER: Silber Joachim
PROVIDER: S-ECPF-GEOD-34242 | biostudies-other |
REPOSITORIES: biostudies-other
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