Profiling of p53-responsive genes in human breast cancer cells harboring endogenous ts-p53 E285K

ABSTRACT: The ts-p53 E285K protein is a rare p53 mutant with temperature-sensitive (ts) loss of function characteristics. In cancer cells, which express ts-p53 E285K intrinsically, endogenous wild type p53 activity is reconstituted by appropriate cultivation temperature (permissive condition). At non-appropriate cultivation temperature (restrictive condition) this p53 mutant is inactive. The present study took advantage of this mechanism and employed IPH-926 lobular breast cancer cells and BT-474 ductal breast cancer cells, which both harbor endogenous ts-p53 E285K, for the transcriptional profiling of p53-responsive genes. This new approach eliminated the need for genetic modification or cytotoxic stimulation to achive a p53 response in the cells being investigated . Three subseqent passages of IPH

ORGANISM(S): Homo sapiens

SUBMITTER: Christgen M 

PROVIDER: S-ECPF-GEOD-35006 | biostudies-other | 2012 Nov

REPOSITORIES: biostudies-other