Transcription profiling of human oral sqamous cell carcinoma patients vs normal samples to associate gene expression profiles and tumor invasion

ABSTRACT: There are limited studies attempting to correlate the expression changes in oral squamous cell carcinoma with clinically relevant variables. We determined the gene expression profile of 16 tumor and 4 normal tissues from 16 patients by means of Affymetrix Hu133A GeneChips. The hybridized RNA was isolated from cells obtained with laser capture microdissection, then was amplified and labeled using T7 polymerase-based in vitro transcription. The expression of 53 genes was found to differ significantly (33 upregulated, 20 downregulated) in normal versus tumor tissues under two independent statistical methods. The expression changes in four selected genes (LGALS1, MMP1, LAGY, and KRT4) were confirmed with reverse transcriptase polymerase chain reaction. Two-dimensional hierarchical clustering o

ORGANISM(S): Homo sapiens

SUBMITTER: Toruner GA 

PROVIDER: S-ECPF-GEOD-3524 | biostudies-other | 2004 Oct

REPOSITORIES: biostudies-other