Vimentin DNA Methylation Predicts Survival in Breast Cancer

ABSTRACT: Purpose:The Vimentin gene plays a pivotal role in epithelial-to-mesenchymal transition (EMT) and is known to be over-expressed in the prognostically poor basal-like breast cancer subtype. Recent studies have reported Vimentin DNA methylation in association with poor clinical outcomes in other solid tumors, but not in breast cancer. We therefore quantified Vimentin DNA methylation in breast tumors and matched normal pairs in association with gene expression and survival in a cohort of 83 breast cancer patients. Materials and Methods:Vimentin methylation was quantified in 14 breast cell lines, 83 breast tumors, and 57 matched normal pairs using MALDI-TOF mass spectrometry. Gene expression data via qRT-PCR in cell lines, and oligo microarray data from breast tissues was correlated with perce

ORGANISM(S): Homo sapiens

SUBMITTER: Ulirsch J 

PROVIDER: S-ECPF-GEOD-35629 | biostudies-other | 2013 Jan

REPOSITORIES: biostudies-other