Whole Transcriptome RNA-seq Analysis: the Tumorigenesis and Metastasis of Melanoma

ABSTRACT: To better understand the tumorigenesis and metastasis of human metastatic melanoma(MM) , we analyzed the transcriptomes of three cell lines that represent the three distinct stages of MM pathogenesis: the normal stage (HEMn), the onset of MM (A375), and the metastasis stage (A2085). Using the next generation sequencing (NGS) technology, we detected unsymmetrical expression of genes particularly in Chr9, 12, and 14 among three cell lines, an indication of the involvement of these genes in the tumorigenesis and metastasis of MM. These genes were assigned into 41 categories by their expression patterns and their biological functions were subsequently analyzed by Ingenuity Pathway Analysis software. In the top category associated with cancer, HIF1A, IL8, TERT, ONECUT1 and FOXA1 had direct inte

ORGANISM(S): Homo sapiens

SUBMITTER: Wang Shaobin 

PROVIDER: S-ECPF-GEOD-35704 | biostudies-other |

REPOSITORIES: biostudies-other