A novel proteomic approach reveals GREB1 as an Estrogen Receptor co-factor

ABSTRACT: Methods for identifying protein-protein interactions have mostly been limited to tagged exogenous expression approaches. We now establish a rapid, robust and comprehensive method for finding interacting proteins using endogenous proteins from limited cell numbers. We apply this approach called ‘Rapid IP-Mass Spectrometry of Endogenous proteins (RIME)’ to identify ER, FoxA1 and E2F4 interacting proteins in breast cancer cells. From small numbers of starting cells, we find a comprehensive collection of known ER, FoxA1 and E2F4 targets, plus a number of novel unexpected interactors. One of the most ER (and FoxA1) associated interactors is GREB1, an estrogen induced gene with almost no known function. We apply RIME, in parallel with ER ChIP-seq, to identify ER protein interactors and ER bindin

ORGANISM(S): Homo sapiens

SUBMITTER: Menon Suraj 

PROVIDER: S-ECPF-GEOD-37386 | biostudies-other |

REPOSITORIES: biostudies-other