MiR-27a-3p in peripheral blood mononuclear cells as a potential marker for pancreatic cancer screening

ABSTRACT: Background & Aims: MicroRNAs have been shown to offer great potential in the diagnosis of cancer. We aimed to identify microRNAs in peripheral blood mononuclear cells (PBMCs) for diagnosing pancreatic cancer (PC). Methods: PBMCs microRNA expression was investigated in three independent cohorts including 352 participants (healthy, benign pancreatic/peripancreatic diseases (BPD), and PC). First, we used sequencing technology to identify differentially expressed microRNAs in 60 PBMCs samples for diagnosing PC. Quantitative reverse-transcriptase polymerase chain reaction assay was then applied to evaluate the expression of selected microRNAs. A logistic regression model was constructed using an independent cohort. Area under the receiver operating characteristic curve (AUC) was used to evaluat

ORGANISM(S): Homo sapiens

SUBMITTER: wang xiaolin 

PROVIDER: S-ECPF-GEOD-37710 | biostudies-other |

REPOSITORIES: biostudies-other