MicroRNA-182-5p targets a network of genes involved in DNA repair

ABSTRACT: MicroRNAs are non-coding regulators of gene expression, which act by repressing protein translation and/or degrading mRNA. Many have been shown to drive tumorigenesis in cancer, but functional studies to understand their mode of action are typically limited to single target genes. In this study we use synthetic biotinylated miRNA to pull down endogenous targets of miR-182. We identified over 1000 genes as potential targets of miR-182, most of which have a known function in pathways underlying tumour biology. Specifically, functional enrichment analysis identified components of both the DNA damage response pathway and cell cycle to be highly represented in this target cohort. Experimental validation confirmed that miR-182 mediated disruption of the homologous recombination (HR) pathway is a

ORGANISM(S): Homo sapiens

SUBMITTER: Krishnan K 

PROVIDER: S-ECPF-GEOD-38593 | biostudies-other | 2013 Feb

REPOSITORIES: biostudies-other