GATA3 acts upstream of FOXA1 in mediating ER binding by shaping enhancer accessibility

ABSTRACT: Estrogen Receptor (ESR1) drives growth in the majority of human breast cancers by binding to regulatory elements and inducing transcription events that promote tumor growth. Differences in enhancer occupancy by ESR1, contribute to the diverse expression profiles and clinical outcome observed in breast cancer patients. GATA3 is an ESR1 co-operating transcription factor mutated in breast tumors, however its genomic properties are not fully defined. In order to investigate the composition of enhancers involved in estrogen-induced transcription and the potential role of GATA3, we performed extensive ChIP-sequencing in unstimulated breast cancer cells and following estrogen treatment. We find that GATA3 is pivotal in mediating enhancer accessibility at regulatory regions involved in ESR1-mediat

ORGANISM(S): Homo sapiens

SUBMITTER: Theodorou V 

PROVIDER: S-ECPF-GEOD-39623 | biostudies-other | 2013 Jan

REPOSITORIES: biostudies-other