RNA sequencing of circulating tumour cells implicates WNT signaling in pancreatic cancer metastasis (human data)

ABSTRACT: Circulating tumour cells (CTCs) shed into blood from primary cancers include putative precursors that initiate distal metastases. While these cells are extraordinarily rare, they may identify cellular pathways contributing to the blood-borne dissemination of cancer. Here, we adapted a microfluidic device for efficient capture of CTCs from an endogenous mouse pancreatic cancer model and subjected CTCs to single-molecule RNA sequencing, identifying Wnt2 as enriched in CTCs. Expression of Wnt2 in pancreatic cancer cells suppresses anoikis, enhances anchorage-independent sphere formation, and increases metastatic propensity in vivo. The effect of Wnt2 is correlated with fibronectin upregulation, and it is mediated in part through non-canonical Wnt signaling and suppressed by inhibition of the

ORGANISM(S): Homo sapiens

SUBMITTER: Yu M 

PROVIDER: S-ECPF-GEOD-40174 | biostudies-other | 2012 Jul

REPOSITORIES: biostudies-other