Genome-wide analysis of RNAs translationally regulated upon BRCA1 depletion in human mammary epithelial cells

ABSTRACT: Loss of function of the tumor suppressor BRCA1 (Breast Cancer 1) protein is responsible for numerous familial and sporadic breast cancers. We previously identified PABP1 as a novel BRCA1 partner and showed that BRCA1 modulates translation through its interaction with PABP1. We showed that the global translation was diminished in BRCA1-depleted cells and increased in BRCA1-overexpressing cells. Our findings raised the question whether BRCA1 affects translation of all cytoplasmic cellular mRNAs or whether it specifically targets a subset of mRNAs. In the present study, we investigated which mRNAs are regulated by BRCA1 using a microarray analysis of polysome-associated RNAs from BRCA1-depleted MCF7 cells, a human breast cancer cell line. We isolated mRNAs from the high-molecular-weight polys

ORGANISM(S): Homo sapiens

SUBMITTER: DACHEUX Estelle 

PROVIDER: S-ECPF-GEOD-40730 | biostudies-other |

REPOSITORIES: biostudies-other