The side population of human pancreatic cancer expresses cancer stem cell-associated genes

ABSTRACT: Purpose: To explore the side population (SP) in pancreatic ductal adenocarcinoma (PDAC) for its gene expression profile and its association to cancer stem cells (CSC) and to evaluate the value of genes from its gene signature on patient survival. Experimental design: Side and main population (MP) cells were isolated from 11 human PDAC resection specimens using fluorescence-activated cell sorting (FACS) after Hoechst incubation. Total RNA was extracted for whole-genome analysis. A gene signature for the purified SP (pSP; depleted from immune/endothelial CD45+/CD31+ cells) was developed and validated with the nCounter system on expression data of 78 primary PDAC using Cox regression analyses for disease-free and overall survival. Results: An SP was identified in all PDAC samples. Whole-gen

ORGANISM(S): Homo sapiens

SUBMITTER: Van den Broeck A 

PROVIDER: S-ECPF-GEOD-42404 | biostudies-other | 2013

REPOSITORIES: biostudies-other