Project description:Recent epidemiological studies demonstrate that both active and involuntary exposure to tobacco smoke increases the risk of breast cancer. Little is known, however, about the molecular mechanisms by which tobacco smoke contributes to breast carcinogenesis. To investigate these mechanisms we have analyzed gene expression and methylation in MCF 10A mammary epithelial cells chronically exposed to aqueous cigarette smoke extract (CSE). CSE was prepared weekly and added to the cell cultures at a concentration equivalent to 0.001 cigarettes/ml. Two clones were isolated after 13 weeks of treatment and expanded in the same concentration of CSE for 8 additional weeks. Mock-treated samples were prepared in parallel.

2013-08-12 | E-GEOD-42667 | biostudies-arrayexpress

Effect of cigarette smoke on gene expression in mammary epithelial cells [methylation]

Project description:Recent epidemiological studies demonstrate that both active and involuntary exposure to tobacco smoke increases the risk of breast cancer. Little is known, however, about the molecular mechanisms by which tobacco smoke contributes to breast carcinogenesis. To investigate these mechanisms we have analyzed gene expression and methylation in MCF 10A mammary epithelial cells chronically exposed to aqueous cigarette smoke extract (CSE). CSE was prepared weekly and added to the cell cultures at a concentration equivalent to 0.001 cigarettes/ml for 21 weeks. Mock-treated samples were prepared in parallel.

2013-08-12 | E-GEOD-42623 | biostudies-arrayexpress

Cigarette Smoke Enhances the Expression of Profibrotic Molecules in Alveolar Epithelial Cells.

Project description: Not available

| S-EPMC4775036 | biostudies-literature

Epigenomic Alterations and Gene Expression Profiles in Human Respiratory Epithelial Cells Mediated by Hookah and Cigarette Smoke

Project description: Not available

| S-EPMC5946508 | biostudies-literature

Cigarette smoke induces genetic instability in airway epithelial cells by suppressing FANCD2 expression.

Project description: Not available

| S-EPMC2391131 | biostudies-literature