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Human glioma cell line T98G: BAP-PCBP2 RIP vs. BAP-GFP RIP (Control)

ABSTRACT: RIP-Chip analysis of PCBP2 and identification of preferentially associated mRNAs. T98G cells were transfected transiently with BAP-tagged constructs. BAP-tagged proteins were biotinylated in vivo by the co-transfected hBirA enzyme. RNPs were recovered via precipitation with Steptavidin-sepharose beads. Finally, RNAs were purified and analyzed on microarrays. BAP-GFP as control was used in three independent sets of experiments. Two-condition experiment, BAP-PCBP2 vs. BAP-GFP cells. Biological replicates: 3 BAP-PCBP2 replicates, 3 BAP-GFP (Control) replicates

ORGANISM(S): Homo sapiens

SUBMITTER: Han W 

PROVIDER: S-ECPF-GEOD-44048 | biostudies-other | 2013 May

REPOSITORIES: biostudies-other

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