Real-time quantitative PCR analysis of human astrocytomas

ABSTRACT: Pediatric astrocytomas, a leading cause of death associated with cancer, are the most common primary central nervous system tumors found in children. Most studies of these tumors focus on adults, not children. We examined the global protein and microRNAs expression pattern by 2D SDS-PAGE, mass spectrometry (MALDI-TOF) and RT2 miRNA PCR Array System. MicroRNAs analysis revealed for the first time novel microRNAs involved in astrocytomas biology. Interestingly, miR-138 and miR-145 down-regulation appear to be associated with protein over-expression of vimentin and Bax, respectively. In conclusion, our results show that novel proteins and microRNAs altered on pediatric astrocytoma could serve as biomarkers to distinguish between astrocytoma grades. Astrocytoma samples were colected from patie

ORGANISM(S): Homo sapiens

SUBMITTER: Ruiz Esparza-Garrido R 

PROVIDER: S-ECPF-GEOD-44280 | biostudies-other | 2013 Dec

REPOSITORIES: biostudies-other