A three marker signature to distinguish Small cell from large cell neuroendocrine carcinoma: Study at transcriptomic and protein level

ABSTRACT: To identify potential markers for distinction of Small cell (SCLC) from large cell neuroendocrine (LCNEC) carcinoma, we have conducted the gene expression profiling on precisely isolated cancer cells and bronchial epithelial cells from these tumours and normal background lung. After statistical analysis and finding highly differentially expressed genes between these tumour groups, we performed qRT-PCR to delineate markers to test at protein level using immunohistochemistry. Markers showing statistical significance at qRT-PCR level were taken forward to stain tissue microarray sections of SCLC and LCNEC to further delineate markers. This resulted in identification of BAI3, CDX2, VIL1 and CD99 as potential candidates. These markers were then taken forward to stain whole sections of tumour sa

ORGANISM(S): Homo sapiens

SUBMITTER: Bari Muhammad 

PROVIDER: S-ECPF-GEOD-44447 | biostudies-other |

REPOSITORIES: biostudies-other