Gene expression profile of A549 cells from tissue of 4D model predicts poor prognosis in lung cancer patients

ABSTRACT: Tumor microenvironment plays an important role in regulating cell growth and metastasis. Recently we developed an ex vivo lung cancer model (4D) that forms perfusable tumor nodules on a lung matrix that mimics human lung cancer histopathology and protease secretion pattern. We compared the gene expression profile (Human OneArray v5 chip) of A549 cells, a human lung cancer cell line, grown on petri dish (2D), and of the same cells grown in the matrix of our ex vivo model (4D). Furthermore, we obtained gene expression data of A549 cells grown on petri dish (2D) and matrigel (3D) from a previous study and compared the 3D expression profile with that of 4D. Expression array analysis showed 2954 genes differentially expressed between 2D and 4D. Gene Ontology (GO) analysis showed up-regulati

ORGANISM(S): Homo sapiens

SUBMITTER: Mishra DK 

PROVIDER: S-ECPF-GEOD-45142 | biostudies-other | 2014 Feb

REPOSITORIES: biostudies-other