Global analysis of DNA methylation changes during progression of oral tumorigenesis

ABSTRACT: Objectives: Earlier studies involving a priori gene selection have identified promoter regions deregulated by DNA methylation changes in oral squamous cell cancers (OSCCs) and precancers. Interrogation of global DNA methylation patterns for such specimens has not been reported, though such analyses are needed to uncover novel molecular factors driving disease. Materials and Methods: We evaluated global DNA methylation patterns for 30 biopsies obtained from 10 patients undergoing surgical removal of an OSCC or carcinoma in situ (CIS). From a disease field in each patient, we collected i) dysplastic, ii) CIS or OSCC, and iii) adjacent normal biopsies. DNA isolated from each biopsy was profiled for methylation status using the Illumina HumanMethylation27K platform. Results: Our data demonstra

ORGANISM(S): Homo sapiens

SUBMITTER: Towle Rebecca 

PROVIDER: S-ECPF-GEOD-46802 | biostudies-other |

REPOSITORIES: biostudies-other