Transcription profiling of human H460 lung cancer cells to investigate chemokinetic versus chemotactic cancer cells

ABSTRACT: Background; Non-small cell lung cancer is the most common cause of early casualty from malignant disease in western countries. The heterogeneous nature of these cells has been identified by histochemical and microarray biomarker analyses. Unfortunately, the morphological, molecular and biological variation within cell lines used as models for invasion and metastasis are not well understood. In this study, we test the hypothesis that heterogeneous cancer cells exhibit variable motility responses such as chemokinesis and chemotaxis that can be characterized molecularly. Methods; A subpopulation of H460 lung cancer cells called KINE that migrated under chemokinetic (no gradient) conditions was harvested from Boyden chambers and cultured. Time-lapsed microscopy, immunofluorescence microscopy

ORGANISM(S): Homo sapiens

SUBMITTER: Soon Lilian 

PROVIDER: S-ECPF-GEOD-4869 | biostudies-other |

REPOSITORIES: biostudies-other