Unknown

Dataset Information

0

MicroRNA response to Snail overexpression in lung cancer cell lines

ABSTRACT: Analysis of four lung cancer cell lines transfected with a vector expressing the transcriptional repressor Snail versus a vector control. Aberrant Snail expression is known to induce an EMT program in lung cancers. Four lung cancer cell lines (H292, H358, H441, H1437) with stable overexpression of the human SNAI1 gene and vector expressing controls were collected at equal confluency with the miRNeasy Mini kit (Qiagen). One microgram of total RNA was labeled using miRCURY LNA™ microRNA Array Power Labeling kit by the UCLA Clinical Microarray Core. The labeled miRNAs were hybridized to Exiqon miRCURY LNA microRNA Array-6th Generation according to the manufacturer’s instructions. This array includes 927/648/351 human/mouse/rat miRNAs as well as 438 miRPlus miRNAs. The miRNA array slides were scanned using Axon GenePix 4000B scanner (Axon Instruments, Foster City, CA) and processed by using the GenePix Pro 6.0 software (Axon Instruments). The raw data were normalized by using a combination of housekeeping miRNA, spike-in miRNA and invariant endogenous miRNAs.

ORGANISM(S): Homo sapiens

SUBMITTER: Dubinett Steven 

PROVIDER: S-ECPF-GEOD-48922 | biostudies-other |

REPOSITORIES: biostudies-other

Json Xml

Similar Datasets

Genomics MicroRNA response to Snail overexpression in lung cancer cell lines
2013-07-17 | GSE48922 | GEO
Transcriptomics MicroRNA response to Snail overexpression in lung cancer cell lines
2013-07-17 | E-GEOD-48922 | biostudies-arrayexpress
Unknown Expression data from FDFT1 overexpression lung cancer cell lines
| S-ECPF-GEOD-37868 | biostudies-other
Unknown Expression data from Snail over-expressing non-small cell lung cancer cell lines
| S-ECPF-GEOD-16194 | biostudies-other
Unknown MicroRNA profiling of human lung cancers and lung cancer cell lines to identify microRNA expression variability between individuals and cell lines
| S-ECPF-TABM-22 | biostudies-other
Unknown Expression data from G9a overexpression and knockdown lung cancer cell lines
| S-ECPF-GEOD-22810 | biostudies-other
Unknown Expression data from human lung cancer cell lines with NEDD9 overexpression or NEDD9 knockdown
| S-ECPF-GEOD-26846 | biostudies-other
Unknown MicroRNA analysis of breast cancer cell lines
| S-ECPF-GEOD-52802 | biostudies-other
Transcriptomics Expression data from Snail over-expressing non-small cell lung cancer cell lines
2010-05-21 | GSE16194 | GEO
Transcriptomics Expression data from Snail over-expressing non-small cell lung cancer cell lines
2010-06-08 | E-GEOD-16194 | biostudies-arrayexpress